Original Articles
Tapan Seal, Kausik Chaudhuri a
Abstract
Identification and quantification of flavonoids (aesculin, catechin, rutin ,naringin, myrecetin, coumarin, luteolin, quercetin, naringenin, apigenin and kaempferol) in two different solvent extracts (methanol and 80% aq. ethanol) of three wild edible leaves of viz. Houttuynia cordata, Solanum gilo and Solanum kurzii collected from Northeastern region in India has been performed using a reversed-phase high-performance liquid chromatography with photodiode array detector. The chromatographic separation was carried out on Acclaim C 18 column (5 μm particle size, 250 x 4.6 mm) , Dionex Ultimate 3000 liquid chromatograph and detection was carried out at three different wave lengths (272 , 280 and 310 nm) using a mobile phase of acetonitrile and water with gradient elution . The experimental results showed good amount of catechin in the 80% aq. ethanol extract of H. cordata (6.63±0.046 mg/gm dry extract) and also in the 80% aq. ethanol extract of S. gilo (2.70±0.041 mg/gm dry extract). The high percentage of recovery (97-100%), low coefficient of variation ( R2 > 0.99) and low limit of detection (LOD) and limit of quantitation (LOQ) confirm the suitability of the method for simultaneous quantification of flavonoid compounds in the two plants under investigation.