Identification and Biochemical characterization of an acidophilic, detergent stable amylase from Klebsiella species isolated from industrial soil of Punjab region

Pradip Kumar G.1, Reena Singh

Abstract

Amylases are starch saccharifying enzymes, which find numerous applications in industry. In this study, amylase producers were isolated from acidic industrial soil of Phagwara region of Punjab, India. Among the amylase producing isolates, one strain Ssta2 showed significant amylase activity in the plate assay. The enzyme was the most active at pH 4.0 and temperature of 40°C. MgCl2 and CaCl2 elicited enzyme activity whereas HgCl2, CuSO4, FeCl3 and CoCl2 inhibited the enzyme. Further analysis revealed that the Ssta2 amylase was stable in presence of 2% SDS, 2% CTAB and 2% Tween-80. The enzyme bears high promise at an industrial scale because of its robust activity