Original Articles
Uttam Kumar Neerudu, Ravi Burg
Abstract
Urine from the pregnant women was collected and then subjected to estimate the protein content which was found to be 243.73 mg with a biological activity of 50IU/mg. Since, purity of a protein has been directly proportional to its biological activity, the obtained protein HCG was subjected to the solvent precipitation method. Subsequently, the purity increased to 150 IU/mg in content of 81.24 mg. After the salt fractionation method, the protein content 48.89 mg exhibited 250 IU/mg of biological activity. This led to the extraction of crude HCG of 24.36 mg with a biological activity of 500 IU/mg.