High Efficient Isolation and Characterization of Endothelial Cells from Newborn Cord Blood Veins

Manizheh Azhdari, Hossein Baha

Abstract

This study introduces a fast and efficient protocol for isolation of human vascular endothelial cells from veins of newborn cord blood for using in cell and drug therapy. Endothelial cells act as barrier between blood and lower layers. These cells, because of their tight connections allow selective molecules to pass through them and loss of them by several factors causes various diseases in humans. Because many studies are not possible in a living organism, hence isolation of consider cells in vitro are also key factors for in vitro studding. Here in, we have introduced an efficient protocol for isolation and culture of endothelial cells from newborn cord blood veins to achieve high purity endothelial cells (above 98%) for in vitro studding, drug and therapy using. This protocol comprises enzymatic and mechanic cord blood veins dissociation as well as a purification step process using anti-CD31 antibodies conjugated to MACS, which produces a pure endothelial cell population of newborn cord blood veins.