GC-MS analysis of bioactive constituents of Indigofera suffruticosa leaves

Vijisaral Elezabeth D and Arum

Abstract

To characterize the chemical constituents of plant of Indigofera suffruticosa leaves using GC-MS. The shade dried leaves powder was extracted with ethanol by using Soxhlet extractor. The Clarus 500 GC used in the analysis employed a column packed with Elite-1 (100% dimethyl poly siloxane, 30 nm × 0.25 nm ID × 1 μM df) and the components were separated using Helium (1 mL/min) as the carrier gas. The 2 μL sample extract injected into the instrument was detected by Turbo gold mass detector (Perkin Elmer) with the aid of the Turbo mass 5.1 software. The GC-MS analysis provided different peaks determining the presence of nine different phytochemical compounds namely pentadecanoic acid,14-methyl-,methyl ester (5.86%), n-hexanedecanoic acid (9.83%), z-[13, 14- epoxy]tetradec-11-en-1-ol acetate(6.37%), oleic acid(10.43%), 9-octadecenoic acid[z]-,2-hydroxy-1-[hydroxyl methyl]ethyl ester(10.21%), heptanoic acid, docosyl ester(6.28%), octadecanoic acid, 7-hydroxy-, methyl ester(4.89%), 6-octadecenoic acid[z]-(18.47%), and 8-octadecenoic acid, methyl ester(14.97%).The bioactive compounds in the ethanolic extract of Indigofera suffruticosa leaves have been screened using this analysis. Isolation of individual phytochemical constituents may proceed to find a novel drug.