GC-MS analysis of bioactive compounds in phenolic extracts of leaves and flowers of Chrysanthemum cinerariaefolium and their efficacy against larvae of Culexquin quefaciatus Say(Diptera: Culicidae)

Ruqia O. Kareem and Mohammed R

Abstract

The bioactive compounds of phenolic extract of leaves and flowers of (pyrethrum) Chrysanthemum cinerariaefolium have been investigated and evaluated using GC-MS analysis. (Perkin Elmer GC clarus 500) Gas Chromatography - Mass Spectrometry, The mass spectra of bioactive compounds were matched according to National Institute of Standards and Technology (NIST) library. The phenolic extract of leaves contains four compounds, while the flowers extract has 13 compounds, The analysis showed also that3,7,11,15-Tetramethyl-2-hexadecen-1-ol,3-Buten- 2-one, and 4-(2-hydroxy-2,6,6-trimethylcyclohexyl) were found in both leaves and flowers extract. Then bioassay of these two phenolic extract were tested against all larval instar of Cx. quinquefaciatus and showed that the first larval instar was more sensitive than other preceding instars and the mortality percentages of these larval instars were high in phenolic extract of flowers.