Factors affecting isolation of fungi from cuticle of apple fruit

Xue Li, Caixia Wang, Baohua Li

Abstract

The purpose of this article was to compare the effects of surface disinfection, medium component, and sample area on the species richness and colony number of fungi isolated from cuticle of bagging apple fruit. The fungi were separated by the tissue isolation and identified based on the morphological characteristics and 18S rDNA sequence. The 3 different influence factors were tested, and the optimum condition of the isolation and cultivation of fungus were screened out in the present study. It was showed that the treatment without any surface disinfection and sampled using a 9mm hole punch should be the best method to isolate fungi in cuticle of apple fruit. There was no significant difference between different medium components. This attempt was also to provide the basic data for the further development of isolation of the fungi from cuticle of apple fruit.