Original Articles
Xin-Hong Wang, Xue-Mei Li and
Abstract
The total flavonoids from Suaeda glauca Bge leaves were studied using ultrasound-assisted extraction (UAE) in this work, and total flavonoids content (TFC) was measured by UV-spectrophotometric method. The inhibiting effect (DPPH%), reducing power, and inhibiting effect of hydroxyl radical (OH%) of sample extract was also investigated. Three important factors with regard to temperature (30-80°C)，time (10-60 min) and liquid-solid ratio (5-50 ml·g-1) were optimized using RSM for obtaining maximum values of response function. The results showed that the optimal extraction condition was at 45.28℃ of extraction temperature, 53.91 min of extraction time, and 30.34 ml·g-1 of liquid-solid ratio, respectively. Under those conditions, TFC, DPPH%, reducing power and OH% were found to be 121.0 mg RE·g-1, 94.67%, 740.07 mg AA·g-1 DW, and 90.91%, respectively. These results demonstrated that the regression model was obtained, and be capable of accurately estimating the total flavonoids from Suaeda glauca Bge leaves in the field of the deep processing of Suaeda glauca Bge resources, as well as desirable anti-DPPH radical activity, reducing power and anti-hydroxyl radical activity. In addition, high linear correlation between TF and OH% indicated inhibition of hydroxyl radical was attributed to flavonoids compounds.