Expression of quinicacidbio synthesis key gene of Escherichia coli

Lin Weiping

Abstract

Quinic acid is an important compound，it have a wide range of application in many fields. We cloned and expressed the key enzyme of quinic acid metabolism pathway using molecular biology technique, and then increased the number of copies of the key enzymes in E. coli, in order to achieve overexpression of quinic acid to achieve mass production. This method, that improved the target product with enhancing metabolic fluxes, lay the foundation for industrial production for the realization of prokaryotic expression quinic acid.