Original Articles
Mahendra Pratap Chopra*, Papiy
Abstract
The ethyl acetate fraction of methanolic extract of Salvia splendens leaves was evaluated for its wound healing effect in Streptozotocin induced diabetic rats. The wound healing activity was evaluated by both Excision and Dead space wound model. A single i.p. dose Streptozotocin (60 mg/kg) was used to induce Diabetes in rats. In the Excision model, wound of circular area (approx. 500 mm2) was created on the back of each animal. Wound healing parameters like percentage of wound contraction, period of epithelialization were observed by topically administering Mupirocin ointment (2% w/w) as standard and methanolic extract (5% and 10% w/w) of S. splendens . Dead space wounds were inflicted by implanting sterile cotton pellets (10mg each) subcutaneously. 5 mg/kg of Glibenclamide standard and methanolic extract of S. splendens were administered in doses of two strengths (200mg/kg and 400mg/kg). The excision of granulation tissue formed on the implanted cotton pellets was done and parameters like Tensile strength, Hydroxyproline content, and Total protein content were measured. Animals treated with extract showed a significant increase in percentage wound closure, tensile strength, hydroxyproline, and Total protein content with a significant decrease in period of epithelialization and blood glucose levels when compared to untreated animals in a dose dependent manner. A significant wound healing potential was seen in the Ethyl acetate fraction of methanolic extract in diabetic rats which may have been due to the presence of phytochemical constituents such as flavonoids, triterpenes, sterols.