Evaluation of the bactericidal efficacy of different dilutions of tincture of iodine on three bacterial reference strains

Bouaziz Assia, Dib Amira Leila

Abstract

The aim of this study is to evaluate the bactericidal efficacy of a disinfectant (iodine) on three bacterial reference strains which are Pseudomonas aeruginosa (CIPA22); Escherichia coli (CIP 55.30) and Staphylococcus aureus (CECT 59), according to the standard (AFNOR NF T72_150, 1995). The results showed that the Gram-negative Pseudomonas aeruginosa strains (CIPA22) and Escherichia coli (CIP 55.30) are more resistant to iodine. Furthermore, Staphylococcus aureus(CECT 59) is the most sensitive gram-positive strain to this disinfectant. No bactericidal dose has been observed in the dilutions used except for dilution (½) of Staphylococcus aureus(CECT 59). The results of this study were used to classify the disinfectant according to its activity, its vis-à-vis efficiency of Gram negative and positive strains and its bactericidal dose.