Evaluation of Binding Affinity with Cancer Causing Receptors, CT-DNA and Biological Activities of Mixed Ligand Complexes of Some Transition Metal Ions

MR Lokesh, G Krishnamurthy, R

Abstract

The novel complexes [ML1L2Cl2] and [ML1L2Cl]Cl where M = Co(II), Cu(II), Ni(II) and Fe(III), containing bioactive ligands of the type L1 = 4-[(2-hydroxy-6-methoxybenzylidene)amino]cyclohexa-1,5-diene-1-sulfonamide and L2 = 1,10-phenanthroline were synthesized and structurally characterized by elemental analysis, conductivity measurement, IR, UV and 1H NMR spectral studies. The DNA binding affinities of synthesized complexes were studied. It was found that all metal complexes were very good DNA intercalators with very high intrinsic binding constant (Kb) than the classical DNA intercalators. The minimum inhibitory concentration (MIC) values of in vitro antimicrobial and antioxidant activity (DPPH Scavenging assay) indicate that the metal complexes were more active compare to the uncoordinated ligand. The Interaction of complexes with the human estrogen receptor and tyrosine kinase (RTK) showed lowest binding energy than the free ligand and the standard indicate that the complexes have high inhibiting property for cancer causing receptors.