Original Articles
Abdul-Malik Abudunia, MÃÂÂ
Abstract
Calendula arvensis (C.A.) is one of the herbs used in traditional medicine in Morocco , Due to its essential oil it is recognized by its smell and warmth characteristic .This work aims to evaluate the antibacterial activity of C.A. flower extracts , Hexanolic and methanolic extracts were obtained by the method of soxhlet , while aqueous extract was obtained by maceration in cold water. Antibacterial activity screening was conducted by the well diffusion method according to the clinical and laboratory standard institute guidelines using 6 Gram positive and 7 Gram negative bacteria (sensitive and multidrug-resistant) .The results indicated that , the methanolic extracts of C.A. flowers generally showed high inhibitory activity against Gram-positive and Gram negative bacteria except Acinetobacter baumanii , Proteus mirabilis and Listeria monocytogenes, The C.A. flowers hexanolic extract inhibited all bacteria of Gram-positive and Gram negative bacteria except, Staphylococcus aureus MRSA, Streptococcus agalactiae and Acinetobacter baumanii , There were no inhibitory effects of the aqueous extracts against all tested bacterial strains except Rhodococcuss equi and Morganella morganii, MICs values of methanolic extracts were between 12.5-25μg/ml . while MICs of values of hexanolic extracts were between 6.25- 12.5 μg/ml . In conclusion , the obtained results show an antibacterial potency of organic extracts of C.A. flowers. C.A. flowers Hexanolic extracts were bactericidal for most bacteria while methanolic extracts were bacteriostatic .