Establishing a methodology to study the influence of quercetin on the metabolism of the K562/ADM cell line using nuclear magnetic resonance technology

Xi Xia, Changli Shao, Qian Liu

Abstract

The purpose of the present study was to establish an experimental method to study the effects of quercetin on the metabolism of the drug-resistant leukemia cell line K562/ADM using nuclear magnetic resonance (NMR) technology. Drug administration to the cells was performed according to proper experimental design and application. The cells were collected and inactivated by repeated freeze-thaw cycles using liquid nitrogen. Methanol (80%) was used to extract cell metabolites, and a sample concentrator (Termovap, ECOM, Czech Republic) was used to purify the samples. All samples were assessed using 500 MHz NMR (Bruker, Germany) with standard NOESY impulse sequence to obtain 1H-NMR spectra of cell metabolites. The processing method used to assess cell metabolism established in the present study was convenient and led to minimal loss of cell metabolites. The 1H-NMR spectra obtained using NMR technology reliably reflected changes in cell metabolism.