Enzymatic hydrolysis of human-like collagen and molecular weight distribution of hydrolysates

Qing Wang, Pei Ma, Xiaoxuan Ma

Abstract

Process parameters on enzymatic hydrolysis and molecular weight distribution of human-like collagen（ HLC） hydrolysates were investigated. HLC was hydrolysed by trypsin and pepsin, the optimal process parameters were obtained by the single-factor and orthogonal experiments. The molecular weight distribution of hydrolysates (MW) was determined using both SDS-PAGE and Sephadex G25 partition. In the study, the maximum degree of hydrolysis (DH) of HLC hydrolysate obtained by trypsin (temperature 50°C, pH 8.0, HLC concentration 15mg/mL, E/S=80%, 8 h) was 70.9%, MW ranged from 500 to 1 400 Da. While that obtained by the pepsin (temperature 50° C, pH 2.5, HLC concentration=5mg/mL, and E/S=3%, 7 h) was 24.25%, MW ranged from 4000 to 6500 Da.