Efficiency comparison between vectors containing the genomic or complementary DNA sequences of human growth hormone in an animal model of gene therapy

Short Communication

Enio Aparecido Zacarias

Abstract

Our institution has been working with gene therapy models for growth hormone deficiency. We are the use of an in vivo approach in which expression vectors containing the increase hormone (GH) gene are administered in mice, followed via electro transference. In previous research, increased levels of human GH (hGH) in mice serum (~20 ng/ mL) and excessive growth approximation to ordinary mice (lure-up growth) of ~70% for frame weight and of ~eighty% for femur duration had been acquired, the use of a plasmid containing the genomic series (gDNA) of GH with the ubiquitin-C promoter. On the alternative hand, we had an illustration that the complementary series (cDNA) also can have a bonus over gDNA in gene treatment protocols.

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