Original Articles
Zhang Rui, Dai Wei, Yao Zhibin
Abstract
This project compares the dynamics of microbial quantities in the soil to analyze the impacts of diluted wood vinegar on soil microbial characteristics. In particular, the project adopts spraying treatments, respectively injecting 300-fold diluted wood vinegar (P300), 500-fold diluted wood vinegar (P500) and the same amount of water (CK) into three different plots. Then we adopt the phospholipid fatty acid (PLFA) method to measure the total microbial quantities, the total bacterial quantities, and the quantities of fungi and actinomycetes the soil, on the 1st, 3rd, 6th, 10th and the 15th day after the injection. The results showed that bacteria were the major microbial composition in the soil and that the dominant bacteria included bacillus spp. and Gram-positive bacteria. Treatments with wood vinegar at the two concentrations significantly increased the quantity of bacteria in soils and led to a significant increase in the total quantity of microbes. The P300 treatment changed the total number of bacteria mainly by changing the number of dominant bacteria, while the P500 treatment not only had a significant impact on the number of dominant bacteria but also showed strong effects on increasing the numbers of Gram-negative bacteria, anaerobic bacteria, aerobic bacteria and other non-dominant bacteria. There were smaller numbers of fungi and actinomycetes in the soils. While wood vinegar at the two concentrations exhibited certain inhibitory effects on soil fungi, the effects on actinomycetes need to be studied further.