Research Article
Mohammad Shah Hafez Kabir,
Abstract
Background: To identify the therapeutic effects of ethanol extract and its different fractions of Phrynium imbricatum leaves in anthelmintic (in vitro) and to determine their total condensed tannin content. Methods: Leaves of Phrynium imbricatum was extracted with pure ethanol and fractioned with chloroform (CHFPI), petroleum ether (PEFPI), n-hexane (NHFPI) and ethyl acetate (EAFPI), which are tested for anthelmintic activity on aquarium worm Tubifex tubifex by using three concentrations viz., 5, 10 and 20 mg/ml of each. Total condensed tannin content determined based on the procedure of Oyedemi et al. Results: Among the various crude extract, ethanol extract Phrynium imbricatum (EEPI) exhibited strong anthelmintic activity in vitro. Where it paralyzed (3.69 ± 0.25 min; P<0.001) and produced death (14.28 ± 0.44 min; P<0.001) of the Tubifex tubifex at 20 mg/ml dose near the value of the standard, Levamisole (3.3 ± 0.38 min and 6.5 ± 0.76 min) at 1 mg/ml. The content of condensed tannin good at EEPI and its all fractions, but EEPI (168.44 ± 0.87 mg catechin/g) contained highest among them. For both of experiment, activity found as follows, EEPI>CHFPI>EAFPI>NHFPI>PEFPI. Conclusion: These findings suggest that the plant may be a potential source for the development of new anthelmintic and condensed tannin may one of such phytochemical, which exhibit anthelmintic activity