Effects of carbon sources and prebiotics added to growth media on proliferation and survival of Lactobacillus bulgaricus LB6 during freeze-drying

He Chen,Shiwei Chen, Hongli Ch

Abstract

Plackett–Burman design employed to evaluate the effect of carbon sources and prebiotics both on proliferation and survival of Lactobacillus bulgaricus LB6 during freeze-drying. Out of consideration of the optimal carbon sources and prebiotics for proliferation and survival of Lactobacillus bulgaricus LB6 before and after freeze-drying, viable counts and survival rate were detected in the medium containing various carbon sources and prebiotics (Glucose, lactose, maltose, sucrose, inulin, trehalose, mannitol, xylooligosaccharides, fructooligosaccharides, galactooligosaccharides). The results indicated that Trehalose out of the investigated carbon sources and prebiotics could both affect the growth (negative) and survival rate (positive) of Lactobacillus bulgaricusLB6 significantly. In addition, Lactose and Galactooligosaccharides have markedly effect on proliferation and survival (all negative), respectively.