Research Article
Adiela Maria Cortes Cortes, Ma
Abstract
Infections associated with health care are a major public health problem due to the occurrence morbidity and mortality and the burden that is impose to patients, to healthcare workers and to the health systems. According to the previous statement it has been identified a serious problem associated with the biomedical element’s disinfection processes, therefore it was necessary to evaluate the efficacy of twelve enzymatic detergents used for cleaning medical devices in Colombia. A preliminary exploratory study was made to determine the effectiveness of the enzymes present in the twelve enzyme detergents (obtained commercially) by three methods: (i) Formulation and observation, (ii) Bioluminescence, which was used to assess the microbial load that cause a reaction with ATP and (iii) Enzymatic activity where the enzyme is in touch with detergents to produce a detected compound by spectrophotometry. Enzyme activity is evident in most commercial products of disinfection, being majority the activity in the product 7 (P7) (12.2761 mM/mL/h) and lower for the product 4 (1.8150 mM/mL/h). P7 represents more effective performance removing organic matter. In this regard, P1, P2, P6 and P9 products had a lower removal performance relative to others, according to the bioluminescence in response to the ATP detected (RLU).