Original Articles
Praveen Satapute and Basappa K
Abstract
Propiconazole is one of the major triazole fungicide, use to treat the standing crops from the disease like leaf spot, powdery mildew and leaf and stem rust. The present work evaluates the impact of propiconazole on the DNA, RNA and protein contents of propiconazole resistant Pseudomonas aeruginosa PS-4 strain in different concentrations (10, 20 and 30 μg/l) and durations. Additionally, the expression of protein under the stress condition was studied by the SDS PAGE analysis. The results indicate that, the higher concentrations (20 and 30 μg/l) of propiconazole were found to be toxic for nucleic acid and protein composition of PS-4 strain and the induction of different protein spots were observed in a pesticide treated group. Moreover, the DNA, RNA and protein contents in the PS-4 strain was found maximum in 10 μg/l of concentration at 24 hrs, it was further decreased after 72 hrs of exposure to propiconazole. Furthermore, the protein banding pattern was observed to be similar in all the propiconazole treated groups and maximum protein spots were observed at 66 to 44 kDa. Results of present investigation exhibits that, the higher dose of toxicant may cause damage to bacteria. Proteins, however, which were expressed under the stress conditions may have the adverse impact on the bacterial strain to resist under certain stress conditions and helps to gain the resistance over toxicants.