Effect of carbon sources including lactose, glucose and maltose on growth of B. bifidum in the extract of Fructus tribuli

Guowei Shu, Yixin Hui, He Chen

Abstract

In this study, the effect of three kinds of carbon sources including lactose, glucose and maltose on growth of Bifidobacteria bifidum BB01 and BB28 in the extract of Fructus tribuli was studied by measuring optical density at 600nm (OD600) and pH. The concentration of three carbon sources was 0.00%, 1.60%, 1.80%, 2.00%, 2.20% and 2.40%. Results were as follows: addition of glucose, maltose and lactose in the extract of Fructus tribuli could promote the growth of Bifidobacteria bifidum BB01, maltose and lactose added to the extract of Fructus tribuli could significantly promote the growth of Bifidobacterium bifidum BB01 but inhibit the growth of Bifidobacterium bifidum BB28. The optimum carbon source on growth of two Bifidobacteria in the extract of Fructus tribuli was glucose. The optimum concentration of Glucose in the extract of Fructus tribuli was 2.40% for Bifidobacterium bifidum BB01and 2.20% for Bifidobacterium bifidum BB28, respectively.