Research Article
Rujuta D. Patel and Dilip G. M
Abstract
The present manuscript describe simple, sensitive, rapid, accurate, precise and cost effective dual wavelength spectrophotometric method for the simultaneous determination of Torsemide (TOR) and Amiloride HCl (AMI) in combined tablet dosage form. The utility of dual wavelength data processing program is its ability to calculate unknown concentration of components of interest in a mixture containing an interfering component. The principle for dual wavelength method is ―the absorbance difference between two points on the mixture spectra is directly proportional to the concentration of the component of interest. The method was based on determination of Torsemide at the absorbance difference between 258.20 nm and 297.40nm and Amiloride HCl at the absorbance difference between 276.80 nm and 300 nm. The linearity was obtained in the concentration range of 5-25 μg/ml for Torsemide and 5-25 μg/ml for Amiloride HCl. The accuracy and precision of the method was determined and validated statically. The method showed good reproducibility and recovery with % RSD less than 2. Method was found to be rapid, specific, precise and accurate, can be successfully applied for the routine analysis of Torsemide and Amiloride HCl in bulk, and combined dosage form without any interference by the excipients. The method was validated according to ICH guidelines.