Differential early dynamic process of marine eukaryotic microbial community on anti-biofouling and biofouling-enhancing micro/nano surfaces

Yuan Sun and Zhizhou Zhang

Abstract

In this study, marine eukaryotic microbial community was analyzed in the context of its complexity and dynamic process in the early stage of biofouling. Samples were collected from five different surfaces: the control group (coating CG), 0.2% Silicium (Coating A), 2% Carbon nanopowder (Coating B), 0.2% Graphite (Coating C) and 2% Silicium plus 2% Carbon nanopowder (Coating F). These coatings were sea-tested for 11 months for their anti-biofouling performance. Coating A and B don’t have anti-biofouling ability just like coating CG, while coating C and F have excellent anti-biofouling capacity. Single stranded conformation polymorphisms (SSCP) was employed to analyze diversity level and dynamic changes of marine fouling eukaryotic communities adhering to different coatings during different sampling time (day 2, 4, 6, 9 and 14). The results indicated that composition, distribution and diversity level of marine fouling eukaryotic microbial community presented obviously differences as sampling time and coating surfaces changed. Coatings with good anti-biofouling performance tend to have low diversity level and different adhering dynamics of marine fouling eukaryotic microbial community.

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