Original Articles
Hiteksha Panchal, Aeshna Amin,
Abstract
A simple, rapid and precise high-performance thin-layer chromatographic method was developed for quantitative simultaneous estimation of Apigenin and Luteolin in aerial part of Achillea millefolium, family Asteraceae. Separation was performed on silica gel 60 F254 HPTLC plates using toluene: ethyl acetate: formic acid (6:4:0.3 v/v/v) as mobile phase for elution of marker from extract. The determination was carried out using densitometric absorbance-reflection mode at 254 nm for Apigenin and Luteloin. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 200-1200 ng/spot for apigenin and 500-3000 ng/spot for luteolin. For apigenin, the limit of detection was found to be 71.06 ng/spot while the limit of quantitation was 230.33 ng/spot respectively and for luteolin, the limit of detection was found to be 74.05 ng/spot while the limit of quantitation was 243.72 ng/spot respectively This developed validated method is capable of quantifying and resolving apigenin and luteolin in its plant raw-material.