Development of Method for Quantitative Determination of Bromelain in Gel Formulation

Nana Gorgaslidze

Abstract

Gel of bromelain has anti-inflammatory properties and hence is used for treatment of edema and inflammations. This was developed at TSMU Iovel Kutateladze Institute of Pharmacochemistry. On previous researches we published the data of some properties of papain and bromelain containing gels. The presented work belongs to the development and validation of accurate and sensitive assay method for quantitative determination of bromelain in gel based on UV-spectrophotometric analysis. Elastasin was used as substrates for their analysis. Absorbance was recorded at 595 nm. The developed method is linear, precise and sensitive, Intra- and inter-day measurements. All strategies were approved according to ICH rules and can be received for the standard analysis of bromelain in gel formulations. Bromelain (Br), a proteolytic catalyst removed from the stem of the pineapple, is known to have mitigating action and has been appeared to decrease blood consistency, forestall the total of blood platelets, and improve ischemia-reperfusion (I/R) injury in a skeletal muscle model. We researched the limit of Br to restrain myocardial injury in a worldwide I/R model.

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