Original Articles
Liu Yan, Xiong Wei, Tian Ji, L
Abstract
A novel method combining multi-wavelength high-performance liquid chromatographic (HPLC) fingerprints and quantitative analysis of multi-component by single marker (QAMS) was developed for quality evaluation of Houttuynia Cordata. For QAMS, Chlorogenic acid was selected as markers to work out the relative correction factors (RCF) of other six components including new chlorogcnic acid, cryptochlorogenic acid, rutin, hyperin, isoquercitrin, quercitrin. The forecast conents of six components were caculated by RCF. No significant difference of the conents was found in comparison with the determined data by external standard method in 20 batches, indicating that the RCF have high reliability within their linear ranges and could be used in quality control of Houttuynia Cordata. An enhanced fingerprints was constructed at two wavelengths (326 and 254 nm). All tested samples contained the 12 common peaks, 7 of which were verified, and the similarity of chromatographic fingerprints was from 0.912 to 0.991. The above new method is to be a new quality evaluation pattern for Traditional Chinese medicine．