Original Articles
Mahesh G. Thakare and Mrinalin
Abstract
A simple, accurate, precise and rapid validated stability indicating HPTLC method of Fluindione was successfully developed. This method is based on HPTLC separation followed by UV detection at 285 nm. The separation was carried out on merck TLC aluminium sheets precoated with silica gel 60F254 using chloroform: methanol (9.9: 0.1% v/v) as a mobile phase. Fluindione gave well defined and sharp peak at Rf 0.47 ± 0.02. Calibration curve was linear in range 1000-3000 ng/band. Stress degradation study shows that sample degraded with acid and base hydrolysis, under oxidation, thermal and photolytic stress conditions. The peak purity parameter ensured noninterference by product of degradation. This method can be applied to determination of stability of Fluindione. The suitability of this HPTLC method for quantitative determination of Fluindione was proved by validation in accordance with requirements of ICH guidelines.