Development and validation of a matrix solid-phase dispersion method coupled to high-performance liquid chromatography with ultraviolet detection for the determination of acylamino acid fungicide residues in green chilli

Tentu. Parvathamma., A. Ramesh

Abstract

A simple, sensitive and inexpensive method was developed using matrix solid-phase dispersion (MSPD), together with high performance liquid chromatographic method for determination of acyclamino acid fungicide residues (Metalaxyl and Benalaxyl) in green chilli. The evaluated parameters included the type and amount of sorbent (silica gel, C18 and neutral alumina) and the nature of eluent (ethyl acetate, dichloromethane and acetonitrile).The best results were obtained using 1.0 g of green chilli sample, 1.0 g of C18 as sorbent and 20ml of ethyl acetatedichloromethane (1:1, (v/v)).The method was validated using in green chilli samples spiked with fungicides at different concentration levels (0.03 and 0.3 µg/mL). Average recoveries (using each concentration six replicates) ranged 90-98%, with relative standard deviations less than 2%, calibration solutions concentration in the range 0.01-2.0 µg/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.01 µg/mL and 0.03 µg/mL respectively.