Determination of Total Phenols and Flavonoid Content of Bryonia laciniosa by Spectrophotometric Method

Shashikala M, Mamata Shah and

Abstract

Bryonia laciniosa Linn. belongs to the family of Cucurbitaceae is one of well known traditional drug. Many pharmacological activities have been reported from this plant including antioxidant activities. As polyphenols are responsible for antioxidant activities, it led to determine the total phenols and flavonoid content in Bryonia laciniosa. The content was estimated in methanol extract of leaves and stem by spectrophotometric method. Gallic acid and quercetin were used as standards for the calibration plot of phenols and flavonoid respectively. The total phenolic content was found to be high in the leaves, 9% than the stem 4%. The total flavonoid content was maximum, 6% in leaves and stem contains the minimum, 2%. As per the authors this is the first report of estimation of total phenols and flavonoid in Bryonia laciniosa.