Determination of the total phenolic and flavonoid contents in Eupatoriurn odoraturn, as well as their antioxidant activities

Qin Deng, Xu Zhang, Fengxia Li

Abstract

Eupatoriurn odoratum aerial parts were extracted with ethanol using ultrasonic-assisted extraction (UAE). Total phenolic content (TPC) and total flavonoid content (TFC) were used to quantify antioxidant compounds with the values of 2.772±0.3 mg GAE (Gallic acid equivalents)/g DW and 7.085±0.2 mg of RT (Rutin)/ g DW. The activities of scavenging 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6 -sulphonicacid) (ABTS) radicals, hydroxyl radicals, ferric ion reducing power and ferrous ion-chelating activity were analyzed, with IC50 values of 3.00, 1.08, 1.18, 0.14 and 3.16 mg/ml, respectively. The study offers theoretic basis for pharmaceutical utilization of the medicinal plant E. odoratum.