Research Article
Narendra M Gowekar and Shailes
Abstract
RP-HPLC method has been developed and validated for estimation of Nimorazole from pharmaceutical dosage form. A chromatographic separation was accomplished on ODS C18 column (100 mm X 4.6mm, 5 μm)with mobile phase consisting of acetonitrile: water (70: 30% v/v)and ultraviolet detection at 297 nm are used for the determination. Under these conditions, the studied Nimorazole elute at 1.60 ± 0.02 min. at a 1 mL/min flow rate. Linearity of Nimorazole was found in the concentration range of 5-30 μg/mL. The LOD and LOQ were found to be 0.2 μg/mL and 0.6μg /mL, respectively. The mean percent recovery of Nimorazole was found to be 99.78 %. All validation parameters were within the acceptable range. Hence, the proposed method can be useful for routine analysis of Nimorazole in pharmaceutical dosage form.