Review Article
Daniel Lee John Bunker
Abstract
The use of gene therapy is rapidly gaining ground in the field of immunotherapy and the correction of monogenetic disorders. An ongoing challenge to the successful implementation of gene therapy to human subjects remains safe and efficient transfer of the plasmid to the host. Strategies for gene transfer include the use of viral vectors or synesthetic transfection systems incorporating physical, chemical, or electrical methods. While viral vectors remain the standard for transduction efficiency, synthetic systems offer potential safety advantages from an immunological perspective.