Controlled hydrolysis studies of the diterpene glycosides rebaudioside D, and rebaudioside E of Stevia rebaudiana Bertoni

Venkata Sai Prakash Chaturvedu

Abstract

Controlled hydrolysis studies of the two sweet diterpene glycosides of Stevia rebaudiana Bertoni namely 13-[(2-O- β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid-[(2-O-β-Dglucopyranosyl- β-D-glucopyranosyl) ester (rebaudioside D, 1) resulted in the formation of rebaudioside A, whereas 13-[(2-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid-(2-O-β-D-glucopyranosyl-β-Dglucopyranosyl) ester (rebaudioside E, 2) yielded stevioside and steviolbioside. The structures of the isolated compounds were characterized on the basis of spectroscopic data as well as comparison with standard compounds.