Original Articles
Wu Guiling
Abstract
Flavonoids from Trollius chinensis Bunge.(Flos Trollii) were extracted by different method were investigated in the present study. Simultaneous determination of two flavonoids(orientin and vitexin) was accomplished by a useful, rapid and simple HPLC/DAD-ESI-MS method. A central composite design (CCD) combined with response surface methodology(RSM) was used to study the effects of extraction time, temperature and liquid to solid ratio on the extraction yield of vitexin and orientin from Trollius chinensis Bunge. The optimum extraction conditions found by maximizing the dependent variables were time of 70min, temperature of 48℃and liquid to solid ratio of 16g/mL, where 1.87% yield was predicted. The results are valuable for further utilizing and development Trollius chinensis Bunge. as a medicinal plant.