Original Article
Liu P, Gao X, Guo X, Wang T, Y
Abstract
The aim of this study was to clone Apx IVA gene of Actinobacillus pleuropneumoniae (APP), to construct cloning vector PMD18-T-APXIV, and finally to perform a bioinformatical analysis after sequencing. This experiment was based on the Apx IVA gene sequence (FJ848574.1), and porcine contagious pleural pneumonia DNA was used template to amplify of Apx IVA fragment by PCR, constructed by TA cloning. The recombinant plasmid pMD18-T-Apx IV was sequenced and followed by Primer 5, DNAMAN and other software for a bioinformatical analysis. The results showed that the amplified length of Apx IVA was 972 bp and PMD-18-T-Apx IV (972 bp) was obtained, which shared the highest sequence similarity (98%) in nucleotide. This study provides a useful reference for the prokaryotic expression and antibody preparation of APP.