Original Articles
Giang Vo Le Duy and Tu Nguyen
Abstract
Finding out the source reducing cholesterol is essential. In the study, Streptococcus thermophillus was cultured in MRS medium. After precipitation with 60% ammonium sulfate, the obtained precipitant was dissolved in distilled water and used to react with cholesterol oleate for 20 minutes at 37oC and then titrated with 1M NaOH and by using phenolphtalein as an indicator till the permanent red color appeared. The tested samples were reacted with cholesteryl oleate and then were run on thin layer chromatography (TLC) with solvent system and then were detected by spraying with 20% perchloric acid and then dried. Finally, the test was observed under ultraviolet (UV) at the wavelength of 230nm. The result reported that Streptococcus thermophillus could produce cholesteryl esterase.