Original Articles
Ali Mohamed El-Hagrassi, Abeer
Abstract
The aim of the present work is to investigate the phenolic profile and the non-polar contents of Abelmoschus esculentus L. leaves as well as, studying the cytotoxic activity of three tested extracts. The chemical compounds of the defatted aqueous methanolic extract of Abelmoschus esculentus L. leaves were analyzed using liquid chromatography-electrospray ionization mass spectrometry technique (LC-ESI-MS) by which twenty two compounds can be identified and classified as seven phenolic acids and their derivatives, with fifteen flavanol derivatives. Petroleum ether fraction of Abelmoschus esculentus L. leaves was analyzed by gas chromatography-mass spectrometry (GC/MS), characterized 31 compounds. Cytotoxic activity was evaluated by using MTT assay against four carcinoma cell lines: HELA, MCF, PC3 and HepG2 (cervix, breast, prostate and liver carcinoma cell lines, respectively).