Research Article
Dehui Lin, Nan Wang, Zhixi
Abstract
Gluconoacetobacter hansenii CGMCC3917 (M438), isolated from inoculums of strain J2 treated by high hydrostatic pressure, has strong ability of producing cellulose as more than three times as that of its initial strain J2. In this paper, in order to further study the effects of high hydrostatic pressure treatment on characterizations of strain J2 on the basis of previous study, properties of these two strains were examined and compared. The results indicated that the mutant strain M438 and its initial strain J2 had different Phenotypic Characterizations in liquid seed medium. The fermentation parameters showed that cell growth rate of strain M438 was relatively higher than that of strain J2, namely, residual sugar, residual nitrogen and acidity of strain M438 were less than that of strain J2. Furthermore, water holding capacity (WHC) and water release rate (WRR) of bacterial cellulose (BC) membranes produced by M438 were both better than those of BC membranes produced by J2. However, SEM imagines suggested that there was no evidence difference in microstructure of BC membranes. Additionally, FT-IR also showed no difference between BC membranes produced by strain M438 and its initial strain J2.