Characterization of GA3 Insensitive Reduced Height Mutant of Emmer Wheat Var. NP200 (Triticum Dicoccum)

Suman Bakshi, Nayeem KA, Bhagw

Abstract

A γ-ray induced semi-dwarf mutant was obtained in tall emmer wheat variety NP200. The mutant was insensitive to externally applied gibberellin. An allele specific marker for dwarfing gene Rht-B1b was used to check the status of dwarfing gene in the mutant, semi dwarf and tall emmer and semi dwarf durum varieties. The primer showed amplification of Rht-B1b gene in semi dwarf durum and emmer varieties. The parent NP200 showed presence of wild type allele (Rht-B1a) with the primer pair BF-WR1. All semi dwarf emmer varieties showed a band of 237bp with primer pair BF-MR1showed presence of Rht-B1b. However, the mutant showed absence of amplification for both Rht-B1a and Rht-B1b alleles with respective primer pairs. The results indicated that the reduced height mutant carried a mutation different from the known allele Rht-B1b.