Research Article
Nisha Sharma, Prakash Chandra
Abstract
The aim of the study was to investigate the biosafety of various fractions of the leaves of P. phoenicea and I. carnea by using brine shrimp lethality bioassay. Evaluation of brine shrimp toxicity assay was carried out for various fractions of leaves of P. phoenicea and I. carnea. None of extracts of P. Phoenicea were found to be toxic upto a dose level of 600 μg/ml. However, the chloroform extract was found to be weakly toxic with LC50 659.8 μg/ml. The various fractions of Ipomoea carnea hexane (LC50 141.4 μg/ml), chloroform (LC50 211.28 μg/ml) and ethyl acetate (LC50 307.28 μg/ml) showed significant toxicity. In the assay the positive control showed LC50 less than 100 μg/ml. The study establishes an addition to the scientific literature, although detailed investigations for the pharmacological activities and active ingredients could provide lead molecules to natural products discovery.