Original Articles
V. Santhi and Anita Kannagi
Abstract
The present study has been aimed to ascertain the antimicrobial activity of extracts from Purpura persica against various pathogenic bacterial and fungal strains using the agar disc diffusion method and the most probable antimicrobial compound by GC-MS study. Crude and eluted fractions were assayed for anti microbial activity against six human bacterial pathogens viz Vibrio cholerae, Salmonella typhi, Shigella flexneri, Escherichia coli, Mycobacterium tuberculosis and Pseudomonas fluorescens and three fungal pathogens viz. Candida albicans, Aspergilus flavus and Actinomyces sp. Maximum inhibition zone were obtained against S. typhi , S.flexneri V.cholerae and E.coli. Of the five column chromatographic fractions, maximum number of pathogens was inhibited by F3 followed by F2 and F1 respectively. Maximum effects of crude extracts of P. persica on fungi were exhibited by chloroform extract. Among the fractions, F2 and F3 showed very good activity against the tested fungi ( C. albicans (19mm) and Actinomyces sp.). The GC/MS study of P.persica revealed the probable antimicrobial compounds such as a steroid compound with chloridate cholest-5-en-3-01(3a) - carbano chloridate and a chloride compound 9, 12-octadecadienoyn chloride (z, z), in F2 fraction and in F3 fraction six antimicrobial compounds were identified such as a phenolic compound eugenol, three Plasticizer compounds, dibutyl phthalate, 1,2- Benzenedicarboxylic acid, diisooctyl ester and Phthalic acid, bis (7-methyloctyl) ester, and a steroid cholest-5-ene, 3-bromo-(3a).