Original Articles
S. Naga Gayatri and V. M. Biju
Abstract
Pirfenidone is an Anti-fibriotic agent, effective in models of Pulmonary and lung fibrosis. It is available as oral and topical preparations. In this study, a rapid, reliable and simple assay method has been developed by using UV – Spectrophotometer. The assay of pirfenidone is based on measuring the absorbance at 220.80 nm wavelength. Pirfenex (A), Pirfetab(B), Fibridone (C) are the three different brands obtained from market for analysis. The concentration range was from 50 -3.125 ppm i.e. solutions of 50 ppm, 25 ppm, 12.5 ppm, 6.25 ppm, 3.125 ppm of each brands were prepared and analysed. The % Assay is calculated and the squared correlation co- efficient for different brands were found to be 0.999 which are with in limit. Hence we can conclude that the method can be applied for fast, routine Quality control quantitative analysis of Pirfenidone formulation. The pirfenex(A) and fibridone(C) showed nearly same % Assay values of 101.26%, 101.00 %. The other brand i.e. pirfetab (B) showed its % Assay value as 100.18 %. All the brands % Assay values were found to be with in limit as per Indian Pharmacopeia.