Research Article
Farrukh Jamal, Sangram Sing
Abstract
Ammonium sulphate fractionated pointed gourd (Trichosanthes dioica) peroxidase- concanavalin A (PGP-Con A) complex was entrapped into calcium alginate-pectin gel. Catalytic performance of immobilized PGP-Con A complex in dye decolorization was examined on repeated use and reusing after a prolonged period of storage. Immobilized and entrapped peroxidase preparation retained 59.6% of the original activity after a period of 50 d. Entrapped PGPCon A complex decolorized 91.2% and 82.1% of the initial color from DR19 and dye mixture [DR19+DB9] after 20 d, respectively. Considerable color removal was found even after 120 d and 80 d respectively, of operation of two reactor system and total organic carbon analysis was quite comparable to color loss. This study shows the efficacy, durability and sustainability of using immobilized T. dioica peroxidase in batch and continuous two reactor catalytic system for the removal of synthetic dyes from industrial effluents.