Application of Home-Made Enzyme and Biosurfactant in the Anaerobic Treatment of Effluent with High Fat Content

Jaqueline do Nascimento Sil

Abstract

The SSF lipase production from Penicillium simplicissimum using an agro industrial residue and the biosurfactant from Pseudomonas aeruginosa were employed for a combined pre-treatment of wastewater from poultry processing industry. During the hydrolysis step of the wastewater fat better results were obtained at 34°C with biosurfactant 0.4% (v/v) and liquid enzyme preparation 6.2% (v/v) or at 46°C with biosurfactant 0.1% (v/v) and liquid enzyme preparation 3.8% (v/v). The pre-hydrolysis in two different conditions of enzyme and biosurfactant concentrations at 34°C and posterior anaerobic treatment allowed the COD removal and methane production, while the no hydrolyzed wastewater besides the COD removal, did not show methane production. Those results suggest the importance and efficiency of the pre-treatment with home-made lipases and biosurfactant during the high fat content wastewater pre-treatment.

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