Research Article
Verawati, Mimi Aria, Afdhil Ar
Abstract
Flavonoids may function as an antioxidant by neutralizing free radicals. This study was aimed to determine total flavonoid content and antioxidant activity from fraction of Piladang (Solenostemon scutellarioides (L) Codd) leaf extract. Total flavonoid was performed with colorimetric method while antioxidant activity of fractions were determined by in-vitro method using DPPH assay. The total flavonoid content was expressed in quercetin equivalent for each fraction was 1.75 mg/g in hexane, 9.63 mg/g in ethyl acetate and 16.78 mg/g in butanol. Antioxidant activity was expressed as 50% inhibitory concentration (IC50) in fractions were 364.73 μg/ml in hexane; 29.15 μg/ml in ethyl acetate and 66.21 μg/ml in butanol. The results indicated a direct correlation between the antioxidant activity and the flavonoid content of the fractions. This study confirmed that the ethyl acetate fraction had a highest activity.