Original Articles
Dongmei Liu, Jiwen Sheng, Jie
Abstract
In this paper, an active fraction (TA) was obtained from Athyrium multidentatum (Doll.) Ching rhizome by silica gel and Sephadex LH-20 column chromatography. The total phenolic content in TA was determined by Folin-Ciocalteu colorimetric method. Antioxidant activity of TA was evaluated employing various established in vitro systems. As a result, TA showed stong reducing power, metal chelating ability and superoxide radical scavenging activity. In the reducing power assay, the absorbance of TA increased from 0.698 ± 0.008 to 2.053 ± 0.011 at concentrations of 4 to 12 μg/mL, which was stronger than vitamin C. These findings indicated that TA was excellent antioxidant and might be a promising prophylactic agent for free radical-related disease.