Research Article
Susanta Kumar Rout, Durga Madh
Abstract
To investigate the antimicrobial, antioxidant and wound healing activities of different fractions of the methanolic extracts of the leaves of Ipomoea Carnea was investigated, Antimicrobial activities was carried out by disc diffusion method and MBC, the wound healing activity was carried out using ether anaesthetized rats in two different wound models i.e. Incision and Excision at two different concentrations(5% and 10%w/w). The free radical scavenging activity was studied in vitro by measuring DPPH, Hydrogen peroxide scavengering activity, Superoxide free radical (O2) and Nitric oxide (NO) free radical scavenging activity with reference to standard antioxidant ascorbic acid. The test compounds like crude methanolic extract (both concentrations) and different fractions like chloroform, methanol and ethyl acetate significantly increase in tensile strength and the rate of wound contraction compared to the control and nitrofurazone. Antimicrobial activities were evaluated against different microorganisms namely Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Acinetobacter baumannii, Bacillus subtilis, Enterococcus faecalis, Candida albicans and Aspergilus Niger. In anti microbial study S. aureus, S.epidermidis, P. aeruginosa, P.merabilis, E. faecalis, showed significant activity on diffusion method and MBC respectively. Different fungal strains like C.albicans and A.niger are not inhibited by different test compounds. The different fractions and extract of ipomoea carnea showed remarkable antioxidant activity. The results revealed that the crude methanolic extract, methanol and ethyl acetate fraction produces remarkable wound healing property due to their antimicrobial and antioxidant activities by possessing the active compounds such as flavonoids, terpenes, alkaloids and saponins.