Antimicrobial, Anti-Inflammatory and Anti-Parkinson’s Screening of Imine Analogues through HSP90 Inhibition

M Ganga Raju, L Saritha, Sayan

Abstract

Objective: Heat shock protein90 is one of a group of molecular chaperones responsible for managing protein folding and quality control in cell environment. Method: In this study an attempt is made to explore role of HSP90 in various activities like antimicrobial, anti-inflammatory, anti-Parkinson’s activity. Two test compounds namely S31 and S33 belonging to class HSP90 inhibitors were studied. The antibacterial and antifungal activities were evaluated by disc-diffusion method using ciprofloxacin and fluconazole as standards respectively. The anti-inflammatory activity of the synthesized compounds was screened by carrageenan and formalin induced paw edema model using indomethacin as standard. The anti-Parkinson’s activity of the compounds was screened by reserpine and haloperidol induced Parkinson’s disease model using bromocriptine as standard drug. Results: From the toxicity studies, the animals were found to be safe upto a dose of 2000 mg/kg bd.wt. The results have shown that the two synthesized compounds S31 and S33 exerted significant antimicrobial, anti-inflammatory, anti-Parkinson’s activity. Conclusion: These activities of the compounds might be due to the inhibition of HSP90 there by arresting the subsequent protein disaggregation and degradation. The S31 has shown significant activity over S33.

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