Antibacterial activity of Parkia speciosa Hassk. peel to Escherichia coli and Staphylococcus aureus bacteria

Hasim, D. N. Faridah and D. A.

Abstract

Parkia speciosa Hassk.is one of edible plants that has not cultivated as medicinal plants yet. The aim of this research was to study antibacterial activity of its peel extract to Staphylococcus aureus and Escherichia coli bacteria with gel diffusion (well method). Peel was extracted using n-hexane, ethyl acetate, and ethanol 70%. The concentration used for each solvent was 50, 100, 150, 200, 250, and 300 mg/mL. The result showed that ethyl acetate extract had the highest antibacterial activity with 404,51% of streptomycin’s antibacterial activity against S. aureus , and 279,12% of streptomycin 10 mg/mL antibacterial activity against E. coli. TLC result showed that ethyl acetate extract with 93:7 toluene:ethyl acetate ratio solvent had eight spots under 254 nm UV. Variance of solvents and its concentration also affected significantly (P<0,05) to inhibition areas.